The stereological approach can provide access to the three-dimensional spatial framework of complex vascular beds. In this presentation, the application of this approach to a variety of vascular beds will be illustrated with examples including the nervous system (brain and spinal cord) and the reproductive system (endometrium). These methods allow us to deduce three-dimensional. Stereology has been applied to a wide variety of problems in Biology in fields such as Neurobiology, Reproductive Biology and Cancer Cell Biology. synapses present in a unit volume using stereological methods. In Biology, it provides a spatial framework upon which to lay physiological and molecular information.
The wide applicability of this approach is because it relies on basic facts of geometry and statistics. Holes can also be considered as structures. In the stereological approach, the exact nature or function of the structure is not important. It is an important and efficient tool in many applications of microscopy (such as petrography, materials science, and biosciences including histology, bone and neuroanatomy). This approach allows inference of geometrical parameters such as volume, surface area, number, thickness and spacing. Stereology is a method that utilizes random, systematic sampling to provide unbiased and quantitative data. It may include any macro or microstructure in biology, materials sciences or indeed geology. Measurement along the Z-axis of the microscope was performed with a 0.5 µm precision microcator (Transreut, Germany) connected to the stage. Disectors were generated as succesive focal planes inside a thick segment of adipose tissue (optical disector). The nature of the structure under study in itself does not matter. Mendis-Handagama, 1992 Mayhew and Gundersen, 1996). For example: šađ in pure pre-eclampsia (PE), vinous and fetoplacental vascular volumes and surfaces are similar to those seen in uncomplicated pregnancies but reduced in pure intrauterine growth restriction (IUGR) and in PE+IUGR šbđ trophoblast extrusion is accelerated in PE and IUGR but the latter exhibits reduced CT proliferation and this perturbed steady state leads to smaller trophoblast volumes and surfaces šcđ Oz diffusive conductances alter in various pregnancy complications, including IUGR and PE+IUGR šdđ fetal weight is diminished, butdiffusive transport increases, in placentas from mice exposed to urban air pollution.The main objective of the stereological approach is to make estimations of parameters of geometrical structures using sampled information. Similar processes occur during development of the mouse placenta and at least some arecompromised in human pregnancies. Invited Review HIGHLIGHTED TOPIC Physiological Imaging of the Lung How much is there really Why stereology is essential in lung morphometry Ewald R. INTRODUCTION Stereology, a quantitative anatomical method was developed to correlate structure and function of various organs1 by extrapolating three dimensional structural quantities from simple counts made on two dimensional slice images. Integration of these processes results in changes in total oxygen (OZ) diffusive conductance which match the growing fetal mass. Keywords: Stereology, Small Intestine, Absorptive Surface, Microvillous. Growth is associatedwith increased numbers of cells or nuclei and counts have shown thatvinous trophoblast continuously renews itself via cytotrophoblast (CT) proliferation and recruitment and syncyriotrophoblast (ST) differentiation andextrusion. 2-1, 2-2) Paraquat (1,1'-dimethyl-4, 4'-bipyridylium chloride), a herbicide widely used in Japan, has been the prime cause of fatal intoxication after ingestion, whether acciden-tal or with suicidal intent. Fetoplacental angiogenesis and vinous growth and maturation involve phased changes particularly around mid-gestation. a) Evaluation of paraquat-induced atelectasis Hamster lung in experimental paraquat toxicity (Figs. We have studied whole-organ function (passive diffusion) in the human placenta and quantified various processes at tissue and subcellular levels and in normal and complicated pregnancies.
My researches have applied established methods to study organ function and developed novel methods to study subcellular localization of marker probes. Stereology can provide hard (funcrionally-relevant) quantitative informarion at different levels of 3D structural organization.